There are two approaches 'maintenance feeding' and 'expansion feeding'. Maintenance feedings keep a culture healthy while expansion feedings are for people wanting to produce more dinoflagellates. This 100ml size would be for maintenance feeding.
Maintenance feeding: Add small amounts (5%-10% of total culture volume) once every week or once every 2 weeks. example for a small 300ml/10oz culture add 1oz/25ml every 2 weeks. 1oz~ 2 tablespoons (DinoNutrients 100ml is ideal for maintenance feeding)
Expansion feeding: Add 30-50% total culture volume once every 7 to 10 days. Insure culture and healthy and dense before expansion. Stop or reduce feedings if bioluminescence is significantly diminished after feeding.