Your Bio-Orb is ready to fill. Simply empty the white PyroDino spout pouch into your Bio-Orb.
Do not add the DinoNutrient pouch at this time.
After filling the Bio-Orb:
Once PyroDinos are added to the Bio-Orb or other container it is best to let the new PyroDinos go without feeding for the first week or two. Wait for the PyroDinos to bounce back from the stress of shipping and transfer. And then wait for healthy PyroDinos to aggregate near the top of the Bio-Orb or new container before feeding. Healthy PyroDinos
will aggregate near the top of their container. Do not feed unless you
see a distinct line of PyroDinos near the top of the container. This
is the sign of a healthy PyroDino culture.
When you have a healthy PyroDino culture as described above you may feed every week or every two weeks.
DO NOT ADD TAP WATER OR ANY WATER - EXCEPT FOR DINONUTRIENTS
Wait for one to two week after arrival until feeding.
Add small amounts (5%-10% of total culture volume) once every week or once every 2 weeks. <example for a small 300ml/10oz culture add 1oz/25ml every 2 weeks>
Add 30-50% total culture
volume once every 7 to 10 days. Ensure culture is healthy and dense
before expansion. Stop or reduce feedings if bioluminescence is
significantly diminished days after feeding.
Temperature: 63F to 79F (17C – 26C)
Dinoflagellates can withstand a wider temperature range if the
changes are gradual and light conditions are complimentary. During
periods of warm temperatures (80F+) low lighting is preferred.
NO direct sunlight or any lighting that adds excess heat to the
dinoflagellate culture should be employed. Indirect (naturally
reflective) sunlight is good. LED lighting is great because it produces
minimal heat. Lighting should be similar to having a small dino
culture 4 to 10 feet away from a single 60W light bulb. Try to keep
your culture in one location throughout the day and night.
Bioluminescence will only occur at night in the dark while the dinoflagellates are gently disturbed. Dinoflagellates will not light up during daytime hours! These tiny plankton have a circadian rhythm or internal clock which helps program them to nighttime and daytime.
Bioluminescence depends on these factors (in order of importance):
1) The amount of contaminating light in the room (darker = better).
2) The density and volume of dinoflagellates used. 3) The time the
dinoflagellates have to generate the light producing molecules luciferin
and luciferase into their nighttime cycle. 4) The overall health of
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