GROWPRO PLUS + INSTRUCTIONS
IMPORTANT: Remove the white PyroDino spout pouch from the shipping box once you receive the shipment.
The white PyroDino spout pouch should be stored in a lighted room. Loosen the cap on the pouch or pouches and leave the PyroDino pouch upright in a location that receives daily filtered sunlight and/or artificial light. Moderate temperatures are recommended. 9C to 31C (48F to 88F). Under these simple conditions, the white PyroDino spout pouch can thrive for several weeks, even months. The ideal temperature is 20C (68F).
You may tighten the cap and gently shake the PyroDino pouch at night in the dark to observe the natural light (bioluminescence). PyroDinos will NOT produce light during daytime hours (day cycle), even in a dark room.
PyroDinos are non-toxic but live in DinoNutrients (purified ocean saltwater) which should not be consumed. Drinking PyroDinos is not harmful but drinking saltwater is not recommended in general.
GROWPRO PLUS 1 LITER FLASK
Tip: To prevent contamination, wash your hands and work in a clean environment with little to no wind or air movement.
Your GrowPro Flasks are clean and ready to fill with PyroDinos. Remove the entire screw cap from the Grow Flask. Remove cap from the white PyroDino spout pouches and pour the contents of the white PyroDino spout pouches into the Grow Flask. Do not add any DinoNutrients at this time (blue pouch). Replace the screw cap on the Grow Flask.
PyroDinos require daily filtered sunlight and/or artificial light. We recommend leaving the grow flasks in a single location throughout the day and night where the Grow Flask contents will not overheat due to direct sunlight or intense lighting. Be aware of temperature swings when placing the Flasks near a window or vent.
GROWPRO PLUS LIGHTING
PyroDinos convert light energy to chemical energy via photosynthesis during the daytime (day cycle). PyroDinos do not produce bioluminescence during their day cycle.
The Programmable LED Grow Light
The Grow Light is powered by a USB plug, making it easy to use with a 5V/1000mA USB outlet. The LED Grow Light has three programmable time settings 8 hours, 12 hours, and 16 hours. The Grow Light also has four intensity settings: high, medium-high, medium-low and low.
Wait until the time of day you want your daily light cycle to begin (Day Cycle). This is usually around sunrise or early in the day, but you can choose any Day Cycle you prefer if the Grow Light is the only light illuminating your Grow Flask.
Place the LED Grow Light over and around the Flask with the LED lights facing down.
The PyroDino LED Grow Light can be used as supplemental lighting or as the sole and main light source.
Supplemental lighting: Use a lower intensity (low or medium-low)
>Try to sync the cycle with the natural light in the room.
Main light source: Use a higher intensity and the 12-hour setting.
Choose the time of day to begin the light cycle program. The time you initiate the program will be the time of day the Grow Light cycles ON each day. Changes to the controller or power surges during the program cycle could disrupt and reset the daily cycle.
Top Button + : Increase Brightness (Four Levels)
Second Button - : Decrease Brightness (Four Levels)
Third Button (clock): Light Timer
8 Hour cycle of light = Blue (16 Hours dark)
12 Hour cycle of light = Green (12 Hour dark)
16 Hour cycle of light = Red (8 Hour dark)
Bottom Button: Power Switch ON/OFF
Flask cultures will also fair well with typical indirect sunlight. A location where you would place a houseplant (like an orchid or tropical plant) should be sufficient.
Keep it simple. The main cause of houseplant death is over-care (over watering). The same could be said for PyroDinos. Leave the PyroDino Grow Flask in the same location day and night. Swirling the flask, day or night, is encouraged but not required for optimal health.
Density is best. PyroDinos thrive in a dense culture. Do not dilute your culture (feed your culture = diluting your culture) until you have a dense PyroDino culture that requires the addition of DinoNutrients. Over-diluting your PyroDino culture can be stressful to the culture. Again, these cells prefer density.
Do not add any water to your culture except for DinoNutrients (or sterile artificial seawater)
Avoid contamination and limit evaporation. PyroDinos are slow-growing marine dinoflagellates cells and can quickly succumb to other fast-growing opportunistic algae and bacteria. Dense PyroDino cultures are less susceptible to contamination. This is another reason to maintain a dense PyroDino culture. Be careful when opening the flask and exposing it to the outside environment. Try to keep the cap on the flask at all times. Keep the cap of the flask dry and free of PyroDino culture contact. When removing the cap of the flask (feeding and pouring) be sure to place the cap on a clean surface. You may use a paper towel or cotton pad damp with 70% IPA (isopropyl alcohol) to wipe down working surfaces and the outside of the flask. Do not introduce any IPA into the Grow Flask.
Bioluminescence (natural light)
PyroDinos will only produce natural light during nighttime hours (dark cycle), in the dark while swirled. The natural light is transient and will only occur while the PyroDinos are in motion. New PyroDinos are ‘programmed’ to begin their dark cycle at 5pm PST. But this can and will change (over several days) if the lighting conditions change. PyroDinos have a circadian clock and will ‘learn’ the new lighting conditions and only produce light while in their dark cycle.
PyroDinos will generate their light-producing compounds throughout their dark cycle. The later into the dark cycle, the brighter the light will appear. Bioluminescence is best viewed in a dark area with little to no contaminating light. After sustained motion, the light potential will slowly diminish but can recharge if left still for a half-hour or more.
DinoNutrients (filtered purified seawater with added nutrients).
PyroDinos are grown in DinoNutrients. PyroDinos can survive for several weeks, even months, with nothing but daily light. For optimal long-term health and to expand your culture (grow more PyroDinos) you should add more DinoNutrients (feeding). ‘Feeding’ is akin to feeding a plant, recommended for optimal health but not altogether necessary for survival.